The Intensity Tracing software is a versatile solution for real-time analysis of fluorescence intensity based on single-photon counts over time. Featuring a user-friendly interface and customizable parameters, this software offers a practical and intuitive platform for researchers to interact with, ensuring seamless data analysis and exploration.

KEY SPECS

Real time display of single-photon counts as a function of time
Data export functionality for further and more in-depth analysis
Python and Matlab scripts for plotting and manipulating the acquired data
Automated saving and loading of parameters configuration
Fast software API for data acquisition (Rust, Python)
Supported platform: MS Windows

Download Intensity Tracing Software

Version 2.2 Beta | Click here to download

See the technical documentation SEE FULL CHANGELOG

MAIN FUNCTION

MAIN TECH-SPECS

Time binning of single-photon counts down to 1 µs

Free-running or fixed-time acquisition mode

Simultaneous acquisition of up to 8 input channels

Adjustable time-span window for data visualization down to 1 s

SYSTEM REQUIREMENT

Windows OS (>= Windows 10)

4GB RAM

Multicore CPU

MAIN FEATURES

When paired with our intuitive Intensity Tracing Software, our compact and lightweight FLIM Data Acquisition Card seamlessly sustains a continuous data stream, facilitating multi-channel real-time analysis of single-photon intensity traces with ease and efficiency.

Real-time display of single-photon counts

Effortlessly sustain continuous data stream for real-time intensity tracing analysis with up to 8 simultaneous acquisition channels. Graphically represent streamed data as waves, with x-axis indicating current time and y-axis depicting single-photon counts.

Single-photon Counts per second (CPS)

Toggle the Show CPS option, and gain the flexibility to display real-time average Photon Count per Second (CPS) on active acquisition channel charts. Even when you’re not actively observing the traces, the intuitive real-time CPS indicator remains visible, offering instant insights into signal intensity. Effortlessly stay informed about your experiments, ensuring a comprehensive understanding of your data at a glance.

Data Export

Our Intensity Tracing software comes equipped with a convenient export data feature, allowing you to effortlessly enable or disable the automatic export of raw data displayed on the charts.
By simply toggling the export option and clicking the “Start” button to initiate real-time data acquisition and plotting, the software automatically writes the data to a .bin file

User Guide

Access the technical documentation of the Intensity Tracing software seamlessly from the GUI with the Embedded User Guide button, ensuring uninterrupted focus on your current experiment.

Time Tagger functionality

The Intensity Tracing software provides advanced functionality for exporting data in various formats, including the widely-used time-tagger format. This ensures that users can efficiently manage and analyze time-stamped events captured during experiments or processes. Whether for short or long-term data collection, the time-tagger export capability offers flexibility and compatibility with other analytical tools or systems.

Acquire and Read Data

The software’s graphical user interface (GUI) is highly versatile, allowing users to operate in both acquire and read modalities. In acquire mode, users can capture real-time data seamlessly, while the read modality enables post-experiment analysis of previously recorded datasets. This dual functionality makes it easy to switch between data collection and detailed analysis without needing separate tools.

Input Channel interface type

Select your preferred connection type for acquisition: USB or SMA. When opting for USB, ideal for acquiring single-photon signals from FLIMLABS single-photon SPAD detectors, the software seamlessly activates the USB firmware within the FLIM Data Acquisition Card. Similarly, selecting SMA triggers the SMA firmware for enhanced functionality.

Acquisition parameters

Our Intensity Tracing software offers an interactive experience with seven parameters.

Enabled Channels: Choose up to eight channels for simultaneous data acquisition.
Channel Type: Select between USB or SMA connection for data acquisition.
Bin Width (μs): Set the duration (in microseconds) for accumulating intensity counts.
Update Rate: Choose between LOW and HIGH for chart update frequency.
Free Running Acquisition Time: Toggle between free and fixed acquisition time modes.
Time Span (s): Set the time interval (in seconds) for the visible data range on the x-axis.
Acquisition Time (s): Specify the acquisition duration (in seconds) when free-running mode is disabled.

MAIN FEATURES

When paired with our intuitive Intensity Tracing Software, our compact and lightweight FLIM Data Acquisition Card seamlessly sustains a continuous data stream, facilitating multi-channel real-time analysis of single-photon intensity traces with ease and efficiency.

Real-time display of single-photon counts

Effortlessly sustain continuous data stream for real-time intensity tracing analysis with up to 8 simultaneous acquisition channels. Graphically represent streamed data as waves, with x-axis indicating current time and y-axis depicting single-photon counts.